Skip to main content

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

High throughput single molecule imaging microscope

Ribbon Icon
Harshad Vishwasrao , Ph.D.
Photo of Harsh Vishwasrao
Acting Director

The high throughput single molecule microscope was designed to image large numbers of cell samples in a multi-well format. This microscope can routinely image up to 8 coverslips and 10,000+ cells per acquisition. An included analysis pipeline automatically segments images to identify nuclei, cell bodies, and puncta (RNAs, receptors, etc.) and generates statistics on these puncta (number/cell, co-expression, co-localization, etc.). This microscope is currently located in the Larson Lab and will move to AIM once the facility is operational.

Image Gallery

High throughput single molecule FISH

Figure 1. High throughput single molecule FISH

 

Example of pipeline for single-molecule RNA FISH Analysis

Figure 2. Pipeline for single-molecule RNA FISH Analysis